Induction of the acrosomal reaction in mouse spermatozoa by the solution of pellucid zone from alpaca (Lama pacos L.)

© 2017 Facultad de Ciencias Biológicas UNMSM. Acrosin is one of the principal components in the acrosome, has trypsin-like activity and is secreted after the Acrosome Reaction (RA). The aim of this study was to study the induction of the acrosomal reaction in mouse sperms with homologous-Pellucid Zone and heterologous-Pellucid Zone. Mouse sperms were capacitated by two hours in IVF medium, supplemented with albumin, heparin and synthetic serum to 37°C and 5% CO2, and before they were incubated in absence of ZP and in present of solubilized ZP from mouse (0,78 mg/ml) and alpaca (0,78 mg/ml y 2,35 mg/ml). The RA was evaluated through immunocytochemistry with monoclonal antibodies anti-human acrosin C5F10 within intervals of 1 hour during four hours. The results show a significant increase (p<0,05) in acrosome reaction induced with homologous-ZP and heterologous-ZP.