Interleukin 10 (IL-10) and transforming growth factor beta (TGF-β) expression in intestinal mucosa of alpaca crias (Vicugna Pacos)

Burga C. Cesar, Manchego S. Alberto, Castro S. Gina, Pérez G. Giovanni, Ramírez V. Mercy, Sandoval C. Nieves

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21 days old (group 2) and 26-47-days old (group 3), regardless sex or breed. The midportion of jejunum of each animal was collected; total RNA was extracted and then analyzed by real-time RT-PCR technique using IL-10 and TGF-β specific primers. The relative mRNA expression analysis was done using the comparative 2-δδCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and jejunum of three 11-month-old fetuses were used as calibrators. The results showed that IL-10 mRNA expression levels were 7.21±1.02 (group 1), 13.53±1.26 (group 2), and 18.77±1.48 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). Also, TGF-β mRNA expression levels were 2.18±0.23 (group 1), 3.03±0.18 (group 2), and 4.06±0.15 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). The expression of IL-10 and TGF-β mRNA was age dependent, increasing significantly in older animals.
Idioma originalInglés estadounidense
Páginas (desde-hasta)439-447
Número de páginas9
PublicaciónRevista de Investigaciones Veterinarias del Peru
DOI
EstadoPublicada - 1 abr 2017

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