Expresión de la Interleucina 10 (IL-10) y el Factor de Crecimiento Transformante Beta (TGF-β) en Mucosa Intestinal de Crías de Alpaca (Vicugna pacos)

Burga C. Cesar, Manchego S. Alberto, Castro S. Gina, Pérez G. Giovanni, Ramírez V. Mercy, Sandoval C. Nieves

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The aim of the present study was to evaluate the relative expression levels of cytokines IL-10 and TGF-β in the intestinal mucosa of clinically healthy alpacas from 2 to 47 days old. The animals were classified in three age groups (six animals per group): 2-8 days old (group 1), 10-21 days old (group 2) and 26-47-days old (group 3), regardless sex or breed. The midportion of jejunum of each animal was collected; total RNA was extracted and then analyzed by real-time RT-PCR technique using IL-10 and TGF-β specific primers. The relative mRNA expression analysis was done using the comparative 2-δδCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as housekeeping gene and jejunum of three 11-month-old fetuses were used as calibrators. The results showed that IL-10 mRNA expression levels were 7.21±1.02 (group 1), 13.53±1.26 (group 2), and 18.77±1.48 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). Also, TGF-β mRNA expression levels were 2.18±0.23 (group 1), 3.03±0.18 (group 2), and 4.06±0.15 (group 3) times as expressed by the calibrate group with significant difference between age groups (p<0.05). The expression of IL-10 and TGF-β mRNA was age dependent, increasing significantly in older animals.

Título traducido de la contribuciónInterleukin 10 (IL-10) and transforming growth factor beta (TGF-β) expression in intestinal mucosa of alpaca crias (Vicugna Pacos)
Idioma originalEspañol
Páginas (desde-hasta)439-447
Número de páginas9
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen28
N.º2
DOI
EstadoPublicada - 1 abr 2017

Palabras clave

  • Alpaca
  • IL-10
  • Intestine
  • Real time RT-PCR
  • T regulator
  • TFG-â

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Profundice en los temas de investigación de 'Expresión de la Interleucina 10 (IL-10) y el Factor de Crecimiento Transformante Beta (TGF-β) en Mucosa Intestinal de Crías de Alpaca (Vicugna pacos)'. En conjunto forman una huella única.

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