MLPA followed by target-NGS to detect mutations in the dystrophin gene of Peruvian patients suspected of DMD/DMB

María Luisa Guevara-Fujita, Francia Huaman-Dianderas, Daisy Obispo, Rodrigo Sánchez, Victor Barrenechea, Diana Rojas-Málaga, Alejandro Estrada-Cuzcano, Milana Trubnykova, Mario Cornejo-Olivas, Victoria Marca, Bertha Gallardo, Milagros Dueñas-Roque, Ana Protzel, Carlos Castañeda, Hugo Abarca, Luis Celis, Jorge La Serna-Infantes, Ricardo Fujita

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Background: We report the molecular analysis of the DMD gene in a group of Peruvian patients with Duchenne/Becker dystrophinopathy. This is the first study to thoroughly characterize mutations in this population. Methods: We used the combination of multiplex ligation-dependent probe amplification (MLPA) and sequencing analysis of the DMD gene. We recruited Peruvian patients in 2 years from reference national hospitals. We performed DNA tests in 152 patients, checking first exon deletion/duplication by MLPA, and subsequently, if negative, samples were sequenced to detect point mutations. Results: The average age for diagnosis was 9.8 years, suggesting a delay for timely diagnosis and care. We found causal DMD mutations in 125 patients: 72 (57.6%) exon deletions/duplications (41.6% deletions, 16.0% duplications), and 53 (42.4%) point mutations (27.2% nonsense, 9.6% small indels, and 5.6% splice site). Conclusion: Due to our genetic background, we expected a higher number of novel and recurrent causal mutations in our sample. Results showed 16% of novel mutations, similar to other well-studied populations.

Idioma originalInglés
Número de artículoe1759
PublicaciónMolecular genetics & genomic medicine
Volumen9
N.º9
DOI
EstadoPublicada - sep 2021

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© 2021 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC

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