Modulation of functional activities of the neurotoxin from black widow spider venom

E. V. Grishin; N. H. Himmelreich; K. A. Pluzhnikov; N. G. Pozdnyakova; L. G. Storchak; T. M. Volkova; P. G. Woll

doi:10.1016/0014-5793(93)80803-3

Modulation of functional activities of the neurotoxin from black widow spider venom

E. V. Grishin, N. H. Himmelreich, K. A. Pluzhnikov, N. G. Pozdnyakova, L. G. Storchak, T. M. Volkova, P. G. Woll

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Resumen

We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [¹⁴C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [¹⁴C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

Idioma original	Inglés
Páginas (desde-hasta)	205-207
Número de páginas	3
Publicación	FEBS Letters
Volumen	336
N.º	2
DOI	https://doi.org/10.1016/0014-5793(93)80803-3
Estado	Publicada - 27 dic. 1993
Publicado de forma externa	Sí

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title = "Modulation of functional activities of the neurotoxin from black widow spider venom",

abstract = "We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.",

keywords = "Calcium influx, GABA release, PC12 cell, Rat brain synaptosome, α-Latrotoxin",

author = "Grishin, {E. V.} and Himmelreich, {N. H.} and Pluzhnikov, {K. A.} and Pozdnyakova, {N. G.} and Storchak, {L. G.} and Volkova, {T. M.} and Woll, {P. G.}",

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AU - Grishin, E. V.

AU - Himmelreich, N. H.

AU - Pluzhnikov, K. A.

AU - Pozdnyakova, N. G.

AU - Storchak, L. G.

AU - Volkova, T. M.

AU - Woll, P. G.

PY - 1993/12/27

Y1 - 1993/12/27

N2 - We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

AB - We have studied the action of an α-latrotoxin (α-LTX) complex of two polypeptides (LTX 130 kDa and low molecular weight protein (LMWP) 8 kDa) and the action of a venom fraction containing LTX with excess LMWP on calcium influx into synaptosomes and PC 12 cells as well as on [14C[GABA release from synaptosomes. Both preparations considerably activate calcium influx and stimulate [14C]GABA release from synaptosomes. Preincubation of both preparations with antibodies against a 14 amino acid residue C-terminal peptide of LMWP differentially modulates these effects. Antibodies inhibit induced calcium influx and enhance induced GABA release.

KW - Calcium influx

KW - GABA release

KW - PC12 cell

KW - Rat brain synaptosome

KW - α-Latrotoxin

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U2 - 10.1016/0014-5793(93)80803-3

DO - 10.1016/0014-5793(93)80803-3

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AN - SCOPUS:0027139337

SN - 0014-5793

VL - 336

SP - 205

EP - 207

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Modulation of functional activities of the neurotoxin from black widow spider venom

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