TY - JOUR
T1 - Molecular epidemiology of plasmodium falciparum malaria outbreak, Tumbes, Peru, 2010–2012
AU - Baldeviano, G. Christian
AU - Akinyi Okoth, Sheila
AU - Arrospide, Nancy
AU - Gonzalez, Rommell V.
AU - Sánchez, Juan F.
AU - Macedo, Silvia
AU - Conde, Silvia
AU - Tapia, L. Lorena
AU - Salas, Carola
AU - Gamboa, Dionicia
AU - Herrera, Yeni
AU - Edgel, Kimberly A.
AU - Udhayakumar, Venkatachalam
AU - Lescano, Andrés G.
N1 - Publisher Copyright:
© 2015, Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2015
Y1 - 2015
N2 - During 2010–2012, an outbreak of 210 cases of malaria occurred in Tumbes, in the northern coast of Peru, where no Plasmodium falciparum malaria case had been reported since 2006. To identify the source of the parasite causing this outbreak, we conducted a molecular epidemiology investigation. Microsatellite typing showed an identical genotype in all 54 available isolates. This genotype was also identical to that of parasites isolated in 2010 in the Loreto region of the Peruvian Amazon and closely related to clonet B, a parasite lineage previously reported in the Amazon during 1998–2000. These findings are consistent with travel history of index case-patients. DNA sequencing revealed mutations in the Pfdhfr, Pfdhps, Pfcrt, and Pfmdr1 loci, which are strongly associated with resistance to chloroquine and sulfadoxine/pyrimethamine, and deletion of the Pfhrp2 gene. These results highlight the need for timely molecular epidemiology investigations to trace the parasite source during malaria reintroduction events.
AB - During 2010–2012, an outbreak of 210 cases of malaria occurred in Tumbes, in the northern coast of Peru, where no Plasmodium falciparum malaria case had been reported since 2006. To identify the source of the parasite causing this outbreak, we conducted a molecular epidemiology investigation. Microsatellite typing showed an identical genotype in all 54 available isolates. This genotype was also identical to that of parasites isolated in 2010 in the Loreto region of the Peruvian Amazon and closely related to clonet B, a parasite lineage previously reported in the Amazon during 1998–2000. These findings are consistent with travel history of index case-patients. DNA sequencing revealed mutations in the Pfdhfr, Pfdhps, Pfcrt, and Pfmdr1 loci, which are strongly associated with resistance to chloroquine and sulfadoxine/pyrimethamine, and deletion of the Pfhrp2 gene. These results highlight the need for timely molecular epidemiology investigations to trace the parasite source during malaria reintroduction events.
UR - http://www.scopus.com/inward/record.url?scp=84928015914&partnerID=8YFLogxK
U2 - 10.3201/eid2105.141427
DO - 10.3201/eid2105.141427
M3 - Artículo
C2 - 25897626
AN - SCOPUS:84928015914
SN - 1080-6040
VL - 21
SP - 797
EP - 803
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 5
ER -