Identificación molecular de salmonella typhimurium en cuyes al primer parto mediante la técnica de PCR multiple

Chero O. Ana, Rosadio A. Raúl, Marcelo M. Geraldine, Díaz O. Gerardo, Jiménez A. Ronald, Castro G. Yenny, Maturrano H. Lenin

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

The aim of the study was to identify serotypes of suspicious isolates of Salmonella spp from breeding Guinea pigs in samples collected within the first week of parturition to detect animals carrying the bacteria. The Guinea pigs were clinically normal and reared in a commercial farm in Pachacamac, Lima, Peru. The farm was free of Salmonella outbreaks in the last four years. A total of 272 paired samples consisting in rectal and vaginal swabs per animal were collected and analyzed using standardized microbiological protocols. The DNA was extracted from suspected isolates of Salmonella sp and then these samples were analyzed by multiplex PCR to detect the presence of invA, prot6E and fliC genes which are specific for Salmonella spp, Salmonella Enteritidis and Salmonella Typhimurium respectively. Eight animals (12 swabs) were positive to Salmonella spp. All 12 isolates amplified invA (Salmonella spp), 10 of them amplified the fliC gene (Salmonella Typhimurium) and none the prot6E gene (Salmonella Enteritidis). The results confirmed Salmonella Typhimurium as the predominant pathogen in breeding Guinea pigs at first parturition in the commercial farm.

Título traducido de la contribuciónMolecular identification of salmonella typhimurium and enteritidis in Guinea pigs at first parturition by multiplex PCR
Idioma originalEspañol
Páginas (desde-hasta)679-686
Número de páginas8
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen28
N.º3
DOI
EstadoPublicada - 1 jul 2017

Palabras clave

  • Females at first parturition
  • Guinea pigs
  • Multiplex PCR
  • Salmonella

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