TY - JOUR
T1 - Morphological and molecular identification of Spirocerca lupi (Nematoda
T2 - Spiruridae) found in the Andean fox (Lycalopex culpaeus)
AU - Gomez-Puerta, Luis A.
AU - Carrasco, Johan
AU - Lopez-Urbina, Maria T.
AU - Gonzalez, Armando E.
N1 - Publisher Copyright:
© 2018, Indian Society for Parasitology.
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Lesions compatible with spirocercosis were found in the esophagus and aorta of an Andean fox from Cuzco, Peru. The esophageal and aortic lesions were 5.5 and 1.5 cm in diameter, respectively. A total of 12 adult nematodes (6 males and 6 females) were collected from the esophageal lesion, and all were identified as Spirocerca lupi by morphological and molecular methods. Molecular characterization was performed by analyzing two sources of the cox1 gene, and the sequences were compared with previous S. lupi sequences from other work deposited in GenBank. Analysis of the partial cox1 gene from S. lupi (n = 3) showed 2 haplotypes and had 95–99% nucleotide similarity to previously described sequences. Also, molecular analysis showed that S. lupi is a very diverse group, due to the genetic variability of the partial sequences of the cox1 gene of Spirocerca. This study is the first to report finding of spirocercosis in the Andean fox.
AB - Lesions compatible with spirocercosis were found in the esophagus and aorta of an Andean fox from Cuzco, Peru. The esophageal and aortic lesions were 5.5 and 1.5 cm in diameter, respectively. A total of 12 adult nematodes (6 males and 6 females) were collected from the esophageal lesion, and all were identified as Spirocerca lupi by morphological and molecular methods. Molecular characterization was performed by analyzing two sources of the cox1 gene, and the sequences were compared with previous S. lupi sequences from other work deposited in GenBank. Analysis of the partial cox1 gene from S. lupi (n = 3) showed 2 haplotypes and had 95–99% nucleotide similarity to previously described sequences. Also, molecular analysis showed that S. lupi is a very diverse group, due to the genetic variability of the partial sequences of the cox1 gene of Spirocerca. This study is the first to report finding of spirocercosis in the Andean fox.
KW - Andean fox
KW - Culpeo fox
KW - Cytochrome c oxidase 1 gene
KW - Lycalopex culpaeus
KW - Nematode
KW - Spirocerca lupi
KW - Spirocercosis
KW - Spirurid
UR - http://www.scopus.com/inward/record.url?scp=85047357434&partnerID=8YFLogxK
U2 - 10.1007/s12639-018-1009-8
DO - 10.1007/s12639-018-1009-8
M3 - Artículo
AN - SCOPUS:85047357434
SN - 0971-7196
VL - 42
SP - 449
EP - 454
JO - Journal of Parasitic Diseases
JF - Journal of Parasitic Diseases
IS - 3
ER -