Multiple genetic origins of histidine-rich protein 2 gene deletion in Plasmodium falciparum parasites from Peru

Sheila Akinyi, Tonya Hayden, Dionicia Gamboa, Katherine Torres, Jorge Bendezu, Joseph F. Abdallah, Sean M. Griffing, Wilmer Marquiño Quezada, Nancy Arrospide, Alexandre Mac Edo De Oliveira, Carmen Lucas, Alan J. Magill, David J. Bacon, John W. Barnwell, Venkatachalam Udhayakumar

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

54 Citas (Scopus)

Resumen

The majority of malaria rapid diagnostic tests (RDTs) detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), encoded by the pfhrp2 gene. Recently, P. falciparum isolates from Peru were found to lack pfhrp2 leading to false-negative RDT results. We hypothesized that pfhrp2-deleted parasites in Peru derived from a single genetic event. We evaluated the parasite population structure and pfhrp2 haplotype of samples collected between 1998 and 2005 using seven neutral and seven chromosome 8 microsatellite markers, respectively. Five distinct pfhrp2 haplotypes, corresponding to five neutral microsatellite-based clonal lineages, were detected in 1998-2001; pfhrp2 deletions occurred within four haplotypes. In 2003-2005, outcrossing among the parasite lineages resulted in eight population clusters that inherited the five pfhrp2 haplotypes seen previously and a new haplotype; pfhrp2 deletions occurred within four of these haplotypes. These findings indicate that the genetic origin of pfhrp2 deletion in Peru was not a single event, but likely occurred multiple times.

Idioma originalInglés
Número de artículo2797
PublicaciónScientific Reports
Volumen3
DOI
EstadoPublicada - 2013

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