New Genetic Variants of Leptospira spp Characterized by MLST from Peruvian Isolates

M. Angélica Delgado, Omar A. Cáceres, John E. Calderón, Lourdes Balda, Giovanna Sotil, Manuel J. Céspedes

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Leptospirosis is a zoonotic disease caused by the genus Leptospira, presenting complex and dynamic epidemiology. To determine the genetic variability and its phylogenetic relationship of Leptospira spp isolates from three sources in Iquitos (Peruvian Amazon) from 2002 to 2013, seven MLST genes were analyzed to obtain the Sequence Type (ST) and these sequences were concatenated for phylogenetic analysis. The genetic relationship between STs was determined with the goeBURST algorithm and genetic diversity was determined using DnaSP. Of 51 isolates, 48 were pathogenic belonging to five different species: Leptospira interrogans Nascimento 2004, Leptospira santarosai Feil 2004, Leptospira noguchii Haake 2021, Leptospira borgpetersenii Levett 2021, and Leptospira kirschneri Levett 2021. Of 20 STs identified, 60% corresponded to new genotypes circulating only in Peru. The genotypes ST17, ST37, and ST301 were recorded in rodents and humans. A high intraspecific genetic diversity was demonstrated in L. noguchi. The goeBURST analysis revealed three clonal complexes (CCs) and 16 singletons. The STs were found to show high genetic variability and phylogenetic and goeBURST analysis determined that the genotypes found did not form specific groups according to the source of infection or origin, which confirms the zoonotic potential of these STs in an area highly endemic for leptospirosis.

Idioma originalInglés
Número de artículo4184326
PublicaciónJournal of Tropical Medicine
Volumen2022
DOI
EstadoPublicada - 2022
Publicado de forma externa

Nota bibliográfica

Publisher Copyright:
© 2022 M. Angélica Delgado et al.

Huella

Profundice en los temas de investigación de 'New Genetic Variants of Leptospira spp Characterized by MLST from Peruvian Isolates'. En conjunto forman una huella única.

Citar esto