TY - JOUR
T1 - Occurrence of Giardia duodenalis assemblages in alpacas in the Andean region
AU - Gomez-Puerta, Luis A.
AU - Lopez-Urbina, Maria T.
AU - Alarcon, Virgilio
AU - Cama, Vitaliano
AU - Gonzalez, Armando E.
AU - Xiao, Lihua
N1 - Funding Information:
We are indebted to Dr. Danilo Pezo (Instituto Veterinario de Investigaciones Tropicales y de Altura, UNMSM) for your support in this study. Luis Gomez-Puerta is supported by a training grant Fogarty/NIH (grant D43TW008273 ) and the alpaca work was supported by the International Foundation for Science (IFS grant B/5225-1 ). The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.
PY - 2014/2
Y1 - 2014/2
N2 - In this study, 352 fecal samples were analyzed for G. duodenalis from alpaca mothers and crias from three different areas of highland in Peru. The triosephosphate isomerase (TPI) gene of Giardia was amplified using a nested PCR protocol. Forty-six G. duodenalis-PCR positive samples were sequenced. G. duodenalis assemblage A was the most frequent followed by assemblage E. The former was seen in 37 animals whereas the latter was seen in nine. Most of the assemblage A infections were caused by the A1 subtype of sub-assemblage AI, except for three, which were caused by the A2 subtype of sub-assemblage AI. Assemblage A was found in all three geographic regions, while assemblage E was detected in crias from two regions. Among the four alpaca mothers positive for Giardia, three had assemblage AI and one had assemblage AII. Results of this study indicate that possible zoonotic transmission human to alpacas.
AB - In this study, 352 fecal samples were analyzed for G. duodenalis from alpaca mothers and crias from three different areas of highland in Peru. The triosephosphate isomerase (TPI) gene of Giardia was amplified using a nested PCR protocol. Forty-six G. duodenalis-PCR positive samples were sequenced. G. duodenalis assemblage A was the most frequent followed by assemblage E. The former was seen in 37 animals whereas the latter was seen in nine. Most of the assemblage A infections were caused by the A1 subtype of sub-assemblage AI, except for three, which were caused by the A2 subtype of sub-assemblage AI. Assemblage A was found in all three geographic regions, while assemblage E was detected in crias from two regions. Among the four alpaca mothers positive for Giardia, three had assemblage AI and one had assemblage AII. Results of this study indicate that possible zoonotic transmission human to alpacas.
KW - Alpaca
KW - Cria
KW - Diarrhea
KW - Giardia duodenalis
UR - http://www.scopus.com/inward/record.url?scp=84886554431&partnerID=8YFLogxK
U2 - 10.1016/j.parint.2013.10.003
DO - 10.1016/j.parint.2013.10.003
M3 - Artículo
C2 - 24141208
AN - SCOPUS:84886554431
SN - 1383-5769
VL - 63
SP - 31
EP - 34
JO - Parasitology International
JF - Parasitology International
IS - 1
ER -