Paternity testing using microsatellite DNA in alpacas (Vicugna pacos)

J. Rodriguez, C. Dodd, R. Rosadio, J. C. Wheeler, M. W. Bruford

Resultado de la investigación: Capítulo del libro/informe/acta de congresoCapítulorevisión exhaustiva

2 Citas (Scopus)

Resumen

Ten alpaca and llama microsatellites (Lang et al., 1996; Penedo et al., 1998) were used to evaluate paternity in 47 alpacas registered at the IVITA Research Station in Maranganí, Canchis Province, Cusco, Peru. The microsatellites were amplified in three multiplex reactions and were polymorphic for all samples. Allele numbers varied between 4 and 20, and both allelic frequencies and exclusion probabilities were calculated using Cervus 2.0. All loci, except two, were within the size range published by Lang et al. (1996) and Penedo et al. (1998). The cumulative exclusion probability for the ten loci was 0.9998, and for each multiplex greater than 0.90. The results confirmed paternity in 18 parent-offspring pairs, but in a further 4 cases different adults were identified as parents than those recorded in the registry, demonstrating the necessity of DNA testing to ensure accurate recordkeeping and guarantee the parentage of registered animals.

Idioma originalInglés
Título de la publicación alojadaSouth American Camelids Research
Subtítulo de la publicación alojadaProceedings of the 4th European Symposium on South American Camelids and DECAMA European Seminar
EditorialWageningen Academic Publishers
Páginas273-278
Número de páginas6
Volumen1
ISBN (versión impresa)9789076998985
DOI
EstadoPublicada - 2006
Publicado de forma externa

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