TY - JOUR
T1 - Phenotypic detection of antimicrobial resistance mechanisms of Escherichia coli isolates from enteric infections in pigs from technified farms
AU - Michelle Monterroso, C.
AU - Guillermo Salvatierra, R.
AU - André Sedano, S.
AU - Sonia Calle, E.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved. The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.
AB - © 2019 Universidad Nacional Mayor de San Marcos. All rights reserved. The aim of this study was to detect the antimicrobial resistance mechanisms of 36 Escherichia coli isolates to beta-lactams, quinolones and aminoglycosides using the Kirby-Bauer technique. Thirty-six E. coli isolates from pigs of technified production farms obtained during the 2010-2015 period were used. Fifteen antimicrobials of importance in human and veterinary medicine were tested. The isolates showed resistance mainly to nalidixic acid (89%, 32/36), cloxacillin (83%, 30/36) and amoxicillin-clavulanic acid (69%, 25/36). Only 3% (1/36) were AmpC producers, 42% (15/36) showed a possible mutation in gyrA and 14% (5/36) at least two possible mutations in gyrA or gyrA+parC. In addition, 33% (12/36) showed high probabilities of presence of qnr genes. The enzymes associated to aminoglycoside resistance mechanism were positive in 39% (14/36) to AAC (6’), 28% (10/36) to ANT (2") and 11% (4/36) to AAC (3) IV.
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U2 - 10.15381/rivep.v30i1.15670
DO - 10.15381/rivep.v30i1.15670
M3 - Article
SN - 1682-3419
SP - 455
EP - 464
JO - Revista de Investigaciones Veterinarias del Peru
JF - Revista de Investigaciones Veterinarias del Peru
ER -
