© 2020 Universidad Nacional Mayor de San Marcos. All rights reserved. The aim of this study was to conduct the sanitary characterization of the fishing marketing chain and identify the points of microbiological contamination from landing to sale, in the port of Pucallpa, Ucayali, Peru. Artisanal fishing ends with the landing of wooden boats with motor. The fish are washed in the river water, as well as plastic material and tubs within 150 m of the riverbank. The fish are transferred covered with crushed ice, plastic layers and rice husk. The facilities for sale are made of wood. There are no programs to disinfect or sanitize the area and solid waste is disposed of in a nearby sector. Work clothing is not used by fishermen, but some primary marketers use it. The physicochemical parameters of the water samples were within the normal ranges, but the presence of coliforms, E. coli and Pseudomona sp at high levels was detected. In fish, mesophiles were above the Maximum Permissible Limit (MPL) in catfish meat (Siluriforme spp); E. coli was at low levels in the meat of the three species evaluated (black prochilodus [Prochilodus nigricans], silver mylossoma [Mylossoma duriventre] and catfish); Staphylococcus aureus was present at low levels, but above the MPL in catfish, and Salmonella sp, Vibrio cholerae and V. parahemolyticus were absent. In facilities, furniture and hands of the fihermen was found E. coli and high counts of coliforms. The results show an artisanal management with poor biosecurity in the fishing marketing chain and a moderate microbiological contamination, both in facilities, in fishermen and in fish.