DETERMINACIÓN DEL SEXO POR ADN EN CINCO ESPECIES DE GUACAMAYOS

R. Jacqueline Liza, H. Lenin Maturrano, A. Raúl Rosadio

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

1 Cita (Scopus)

Resumen

Determination of sex in wild birds is crucial in developing conservation plans for threatened species. The absence of sexual dimorphism in birds makes impossible to determine sex based on physical characteristics and sexing has traditionally depended upon surgical methods which are highly stressful for the animals. The present study had the purpose of developing of a noninvasive DNA test for sex determination in macaws (guacamayos). Blood samples from five species (Ara ararauna, Ara chloroptera, Ara macao, Ara militaris y Propyrrhura couloni) were studied. DNA was extracted and specific primers were used to amplify both exons and introns of the chd (chromo-helicase- DNA-binding) gene located on the sex chromosomes of all birds. Females are identified by chd-ZW on the W chromosome and males by chd-ZZ on the Z chromosome. The amplification was carried out by the optimization of the conditions and the PCR cycles. Two fragments of 300-400 bp were detected in female birds and one in males. Sex determined by conventional methods in 31 birds agreed with DNA results. The molecular test was also used to sex 28 macaws of unknown sex, including 11 Propyrrhura couloni.

Título traducido de la contribuciónSex determination by ADN in five macaw species
Idioma originalEspañol
Páginas (desde-hasta)31-36
Número de páginas6
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen19
N.º1
EstadoPublicada - 2008
Publicado de forma externa

Palabras clave

  • CHD gene
  • Macaw
  • P2 and P8 primers
  • Sex

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