Short report: Molecular mass screening to incriminate sand fly vectors of Andean-type cutaneous leishmaniasis in Ecuador and Peru

Hirotomo Kato, Abraham German Caceres Lazaro, Eduardo A. Gomez, Tatsuyuki Mimori, Hiroshi Uezato, Jorge D. Marco, Paola A. Barroso, Hiroyuki Iwata, Yoshihisa Hashiguchi

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

41 Citas (Scopus)

Resumen

Sand flies from the Andean areas of Ecuador and Peru were examined for Leishmania infections by using our recently established molecular mass screening method. Leishmanial minicircle DNA-positive sand flies were detected in 3 of 192 and 1 of 462 samples from Ecuador and Peru, respectively. Sand fly species were identified by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the 18S ribosomal RNA (rRNA) gene, and the positive flies were Lutzomyia (Lu.) ayacuchensis and Lu. peruensis, respectively. Furthermore, cytochrome b and mannose-phosphate isomerase gene sequence analyses identified the parasites from Ecuador and Peru as Leishmania (Leishmania) mexicana and L. (Viannia) peruviana, respectively. Thus, the mass screening method was confirmed to be a powerful tool for sand fly research.

Idioma originalInglés
Páginas (desde-hasta)719-721
Número de páginas3
PublicaciónAmerican Journal of Tropical Medicine and Hygiene
Volumen79
N.º5
DOI
EstadoPublicada - nov. 2008
Publicado de forma externa

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