Simple Sequence Repeat-Based Genetic Diversity and Analysis of Molecular Variance among on-Farm Native Potato Landraces from the Influence Zone of Camisea Gas Project, Northern Ayacucho, Peru

Germán De la Cruz, Tomás Yuret Miranda, Raúl Humberto Blas, Edgar Neyra, Gisella Orjeda

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The Andean native potato is an important food security crop and constitutes a gene pool for potato-breeding. To establish the current state of the native potatoes diversity in the influence zone of the Camisea Gas Project, North Ayacucho, Peru, landraces (n = 144) were collected. Three sampling populations (Anco, Chungui and Ticllas) were resolved using 10 simple sequence repeats (SSRs) markers that amplified 67 polymorphic alleles. Principal component, correspondence and cluster analysis revealed a minimum set of six SSRs to achieve DNA fingerprinting and cost-effective genetic diversity analysis. Analysis of molecular variance (AMOVA), average fixation index (FST = 0.279), and genetic distance index (SB) indicated that the genetic diversity of the native potatoes is high at the intra- and inter-population levels, and each of the three sampling populations constituted closed populations. The three populations were genetically distinct and contained unique genotypes and exclusive alleles. Misidentified native potatoes (10.41%) were found.

Idioma originalInglés
Páginas (desde-hasta)143-161
Número de páginas19
PublicaciónAmerican Journal of Potato Research
Volumen97
N.º2
DOI
EstadoPublicada - 1 abr 2020

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Publisher Copyright:
© 2020, The Potato Association of America.

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