TY - JOUR
T1 - Simultaneous determination of albendazole metabolites, praziquantel and its metabolite in plasma by high-performance liquid chromatography-electrospray mass spectrometry
AU - Bonato, Pierina Sueli
AU - Oliveira, Anderson Rodrigo Moraes de
AU - Santana, Fernando José Malagueño de
AU - Fernandes, Bruno José Dumet
AU - Lanchote, Vera Lucia
AU - Gonzalez, Armando E.
AU - Garcia, Hector H.
AU - Takayanagui, Osvaldo Massaiti
N1 - Funding Information:
The authors are grateful to Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and the National Institutes of Health, USA (Grant NI-51976) for financial support and for granting research fellowship.
PY - 2007/6/28
Y1 - 2007/6/28
N2 - The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.
AB - The analysis of albendazole sulfoxide, albendazole sulfone, praziquantel and trans-4-hydroxypraziquantel in plasma was carried out by high-performance liquid chromatography-mass spectrometry ((LC-MS-MS). The plasma samples were prepared by liquid-liquid extraction using dichloromethane as extracting solvent. The partial HPLC resolution of drug and metabolites was obtained using a cyanopropyl column and a mobile phase consisting of methanol:water (3:7, v/v) plus 0.5% of acetic acid, at a flow rate of 1.0 mL/min. Multi reaction monitoring detection was performed by electrospray ionization in the positive ion mode, conferring additional selectivity to the method. Method validation showed relative standard deviation (precision) and relative errors (accuracy) lower than 15% for all analytes evaluated. The quantification limit was 5 ng/mL and the linear range was 5-2500 ng/mL for all analytes. The method was used for the determination of drug and metabolites in swine plasma samples and proved to be suitable for pharmacokinetic studies.
KW - Albendazole sulfone
KW - Albendazole sulfoxide
KW - LC-MS-MS
KW - Plasma
KW - Praziquantel
KW - trans-4-Hydroxypraziquantel
UR - http://www.scopus.com/inward/record.url?scp=34249940916&partnerID=8YFLogxK
U2 - 10.1016/j.jpba.2006.11.020
DO - 10.1016/j.jpba.2006.11.020
M3 - Artículo
C2 - 17169522
AN - SCOPUS:34249940916
SN - 0731-7085
VL - 44
SP - 558
EP - 563
JO - Journal of Pharmaceutical and Biomedical Analysis
JF - Journal of Pharmaceutical and Biomedical Analysis
IS - 2
ER -