Standardization and validation of qualitative realtime RT-PCR for detection of classical swine fever virus

Agricultura y biología

• Classical swine fever virus 94%
• standardization 69%
• reverse transcriptase polymerase chain reaction 52%
• assays 23%
• fluorescent antibody technique 21%
• 5' untranslated regions 16%
• testing 14%
• Border disease virus 11%
• RNA 9%
• viruses 9%
• Bovine viral diarrhea virus 1 9%
• tonsils 9%
• Rotavirus 9%
• lymph 9%
• complementary DNA 8%
• melting point 7%
• vaccines 6%
• glycoproteins 6%
• sampling 5%
• swine 4%
• synthesis 4%
• tissues 3%

Medicina y ciencias biológicas

• Classical Swine Fever Virus 100%
• Real-Time Polymerase Chain Reaction 47%
• Polymerase Chain Reaction 39%
• Fluorescent Antibody Technique 19%
• 5' Untranslated Regions 17%
• Border disease virus 13%
• Bovine Viral Diarrhea Viruses 10%
• Viruses 9%
• Rotavirus 8%
• Palatine Tonsil 8%
• Lymph 8%
• Viral RNA 8%
• Freezing 7%
• Glycoproteins 6%
• Complementary DNA 6%
• Swine 5%
• Vaccines 5%
• Sensitivity and Specificity 5%
• Virus Diseases 5%
• Temperature 5%
• RNA 5%