The effect of the essential oil from aloysia triphylla britton (lemon verbena) on trypanosoma cruzi in mice

Juan Rojas, Olga Palacios, Sergio Ronceros

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

11 Citas (Scopus)

Resumen

Objectives. To determine the in-vivo anti-Trypanosoma cruzi activity of the essential oil from Aloysia triphylla in mice. Materials and methods. The mice (n = 15) in the study were randomly assigned to the following groups: infected and untreated (G1), infected and treated with benznidazole 100 mg/kg (G2), infected and treated with of Aloysia triphylla essential oil 100 mg/kg (G3), infected and treated with of Aloysia triphylla essential oil 250 mg/kg (G4), uninfected and untreated (G5), and uninfected and treated with 250 mg/kg of Aloysia triphyla (G6). The infection was conducted using 104 blood T. cruzi trypomastigotes and the treatment began on the 8th day post-infection (dpi) through 28 dpi. The parasitemia was determined through optical microscopy every 2 days using 5 μL of blood sampled from the tail. In dpi 14, 21 and 28, the blood sample was obtained from the queue for testing creatine kinase-MB, alanine aminotransferase and creatinine, then animals were euthanized and the heart was removed for histopathological study. Results. The essential oil of lemon verbena produced a signifcant reduction of the parasitemia, 85.4% with the peak at a the dose of 250 mg/ kg. It was also observed a reduction in the number of amastigotes and infammatory infltrates in the heart. The creatine kinase-MB plasma levels also decreased at dpi 28 as a result of such treatment. Conclusions. Under experimental conditions, the essential oil of Aloysia triphylla (lemon verbena) has in vivo anti-Trypanosoma cruzi effect in mice.
Idioma originalInglés estadounidense
Páginas (desde-hasta)61-68
Número de páginas8
PublicaciónRevista Peruana de Medicina Experimental y Salud Publica
DOI
EstadoPublicada - 1 ene 2012

Huella

Profundice en los temas de investigación de 'The effect of the essential oil from aloysia triphylla britton (lemon verbena) on trypanosoma cruzi in mice'. En conjunto forman una huella única.

Citar esto