The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça; Camila S. Palma; Daniele Albuquerque; Gabriela Norma Solano Canchaya; Mariana L. Grassi; Vera L. Epifânio; Eduardo B. de Oliveira

doi:10.1007/978-1-4939-0685-7_22

The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça, Camila S. Palma, Daniele Albuquerque, Gabriela Norma Solano Canchaya, Mariana L. Grassi, Vera L. Epifânio, Eduardo B. de Oliveira

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

4 Citas (Scopus)

Resumen

The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

Idioma original	Inglés
Páginas (desde-hasta)	323-335
Número de páginas	13
Publicación	Methods in Molecular Biology
Volumen	1156
DOI	https://doi.org/10.1007/978-1-4939-0685-7_22
Estado	Publicada - 2014

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abstract = "The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.",

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AU - Faça, Vitor M.

AU - Palma, Camila S.

AU - Albuquerque, Daniele

AU - Solano Canchaya, Gabriela Norma

AU - Grassi, Mariana L.

AU - Epifânio, Vera L.

AU - de Oliveira, Eduardo B.

PY - 2014

Y1 - 2014

N2 - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

AB - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

KW - Cancer

KW - Mass spectrometry

KW - Multiple reaction monitoring

KW - Protein fractionation

KW - SILAC

KW - Secretome

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DO - 10.1007/978-1-4939-0685-7_22

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AN - SCOPUS:84922393306

SN - 1064-3745

VL - 1156

SP - 323

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JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

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The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

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