TY - JOUR
T1 - The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)
AU - Faça, Vitor M.
AU - Palma, Camila S.
AU - Albuquerque, Daniele
AU - Canchaya, Gabriela N.S.
AU - Grassi, Mariana L.
AU - Epifânio, Vera L.
AU - de Oliveira, Eduardo B.
N1 - Publisher Copyright:
© 2014, Springer Science+Business Media New York.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2014
Y1 - 2014
N2 - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.
AB - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.
KW - Cancer
KW - Mass spectrometry
KW - Multiple reaction monitoring
KW - Protein fractionation
KW - SILAC
KW - Secretome
UR - http://www.scopus.com/inward/record.url?scp=84922393306&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-0685-7_22
DO - 10.1007/978-1-4939-0685-7_22
M3 - Artículo
C2 - 24791999
AN - SCOPUS:84922393306
SN - 1064-3745
VL - 1156
SP - 323
EP - 335
JO - Methods in Molecular Biology
JF - Methods in Molecular Biology
ER -