The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça; Camila S. Palma; Daniele Albuquerque; Gabriela N.S. Canchaya; Mariana L. Grassi; Vera L. Epifânio; Eduardo B. de Oliveira

doi:10.1007/978-1-4939-0685-7_22

The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Vitor M. Faça, Camila S. Palma, Daniele Albuquerque, Gabriela N.S. Canchaya, Mariana L. Grassi, Vera L. Epifânio, Eduardo B. de Oliveira

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

4 Citas (Scopus)

Resumen

The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

Idioma original	Inglés
Páginas (desde-hasta)	323-335
Número de páginas	13
Publicación	Methods in Molecular Biology
Volumen	1156
DOI	https://doi.org/10.1007/978-1-4939-0685-7_22
Estado	Publicada - 2014

Nota bibliográfica

Publisher Copyright:
© 2014, Springer Science+Business Media New York.

Acceder al documento

10.1007/978-1-4939-0685-7_22

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{b7ef7f21396b4366a24828e268d7c9db,

title = "The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)",

abstract = "The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.",

keywords = "Cancer, Mass spectrometry, Multiple reaction monitoring, Protein fractionation, SILAC, Secretome",

author = "Fa{\c c}a, {Vitor M.} and Palma, {Camila S.} and Daniele Albuquerque and Canchaya, {Gabriela N.S.} and Grassi, {Mariana L.} and Epif{\^a}nio, {Vera L.} and {de Oliveira}, {Eduardo B.}",

year = "2014",

doi = "10.1007/978-1-4939-0685-7_22",

language = "Ingl{\'e}s",

volume = "1156",

pages = "323--335",

journal = "Methods in Molecular Biology",

issn = "1064-3745",

publisher = "Humana Press Inc.",

}

TY - JOUR

T1 - The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

AU - Faça, Vitor M.

AU - Palma, Camila S.

AU - Albuquerque, Daniele

AU - Canchaya, Gabriela N.S.

AU - Grassi, Mariana L.

AU - Epifânio, Vera L.

AU - de Oliveira, Eduardo B.

PY - 2014

Y1 - 2014

N2 - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

AB - The secretome is a sub-proteome of great interest in several fields of biomedical sciences, especially as a source of diagnostics and therapeutic targets. Proteomics has been contributing significantly to elucidate the secretome of a great diversity of cells, tissues, and organisms, turning profiles of thousands of proteins a usual practice. After elucidation of long protein lists, targeted proteomics also plays important roles in accurate quantification and validation of such secreted proteins. Here we present detailed protocols to explore and quantify the secretome of cancer cells, even though this protocol can be employed to any kind of biological material.

KW - Cancer

KW - Mass spectrometry

KW - Multiple reaction monitoring

KW - Protein fractionation

KW - SILAC

KW - Secretome

UR - http://www.scopus.com/inward/record.url?scp=84922393306&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-0685-7_22

DO - 10.1007/978-1-4939-0685-7_22

M3 - Artículo

C2 - 24791999

AN - SCOPUS:84922393306

SN - 1064-3745

VL - 1156

SP - 323

EP - 335

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

The secretome analysis by high-throughput proteomics and multiple reaction monitoring (MRM)

Resumen

Nota bibliográfica

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto