Toward detection of toxoplasmosis from urine in mice using hydro-gel nanoparticles concentration and parallel reaction monitoring mass spectrometry

Hannah E. Steinberg, Paul Russo, Noelia Angulo, Raúl Ynocente, Cristina Montoya, Andrea Diestra, Cusi Ferradas, Francesca Schiaffino, Edgar Florentini, Juan Jimenez, Maritza Calderón, Vern B. Carruthers, Robert H. Gilman, Lance Liotta, Alessandra Luchini

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

3 Citas (Scopus)

Resumen

Diagnosis of clinical toxoplasmosis remains a challenge, thus limiting the availability of human clinical samples. Though murine models are an approximation of human response, their definitive infection status and tissue availability make them critical to the diagnostic development process. Hydrogel mesh nanoparticles were used to concentrate antigen to detectable levels for mass spectrometry. Seven Toxoplasma gondii isolates were used to develop a panel of potential peptide sequences for detection by parallel reaction monitoring (PRM) mass spectrometry. Nanoparticles were incubated with decreasing concentrations of tachyzoite lysate to explore the limits of detection of PRM. Mice whose toxoplasmosis infection status was confirmed by quantitative real-time PCR had urine tested by PRM after hydrogel mesh concentration for known T. gondii peptides. Peptides from GRA1, GRA12, ROP4, ROP5, SAG1, and SAG2A proteins were detected by PRM after nanoparticle concentration of urine, confirming detection of T. gondii antigen in the urine of an infected mouse.

Idioma originalInglés
Páginas (desde-hasta)461-469
Número de páginas9
PublicaciónNanomedicine: Nanotechnology, Biology, and Medicine
Volumen14
N.º2
DOI
EstadoPublicada - feb. 2018

Nota bibliográfica

Publisher Copyright:
© 2017 Elsevier Inc.

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