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Resumen

A method for determining the sex of DNA samples from alpacas has been developed using amplification of the SRY (Sex-determining Region Y chromosome) gene. New oligonucleotide primers were designed according to the SRY gene partial sequence from guanaco (Lama guanicoe). After electrophoresis, a normal male alpaca showed a single SRY band of 146 bp that did not appear in female alpaca samples. After optimization, the PCR procedure for determination of sex was applied to 10 DNA alpaca samples. The sex (genotypic) as diagnosed by PCR corresponded with anatomical sex (phenotypic) in all cases. This study showed that the present method can be applied for sexing DNA samples from alpacas by PCR amplification of the SRY gene.

Título traducido de la contribuciónA method for sexing alpaca DNA by PCR amplification of the SRY gene
Idioma originalEspañol
Páginas (desde-hasta)203-207
Número de páginas5
PublicaciónRevista de Investigaciones Veterinarias del Peru
Volumen20
N.º2
EstadoPublicada - 2009

Nota bibliográfica

Publisher Copyright:
© 2009 Universidad Nacional Mayor de San Marcos. All rights reserved.

Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.

Palabras clave

  • Alpaca
  • Bioinformatics
  • Molecular diagnosis
  • PCR
  • SRY
  • Y chromosome

Huella

Profundice en los temas de investigación de 'Un método para el sexaje por adn de alpaca amplificando el gen SRY mediante PCR'. En conjunto forman una huella única.

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