Use of ProteinChip technology for identifying biomarkers of parasitic diseases: The example of porcine cysticercosis (Taenia solium)

N. Deckers, P. Dorny, K. Kanobana, J. Vercruysse, A. E. Gonzalez, B. Ward, M. Ndao

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

14 Citas (Scopus)

Resumen

Taenia solium cysticercosis is a significant public health problem in endemic countries. The current serodiagnostic techniques are not able to differentiate between infections with viable cysts and infections with degenerated cysts. The objectives of this study were to identify specific novel biomarkers of these different disease stages in the serum of experimentally infected pigs using ProteinChip technology (Bio-Rad) and to validate these biomarkers by analyzing serum samples from naturally infected pigs. In the experimental sample set 30 discriminating biomarkers (p < 0.05) were found, 13 specific for the viable phenotype, 9 specific for the degenerated phenotype and 8 specific for the infected phenotype (either viable or degenerated cysts). Only 3 of these biomarkers were also significant in the field samples; however, the peak profiles were not consistent among the two sample sets. Five biomarkers discovered in the sera from experimentally infected pigs were identified as clusterin, lecithin-cholesterol acyltransferase, vitronectin, haptoglobin and apolipoprotein A-I.

Idioma originalInglés
Páginas (desde-hasta)320-329
Número de páginas10
PublicaciónExperimental Parasitology
Volumen120
N.º4
DOI
EstadoPublicada - dic. 2008

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